Effects of three different inducers on plant morphologic characteristics and biochemical parameters of Fuxiangjiayu, a strain of Capsicum annuum L., were studied. The results showed that inducer treatment under suitable concentration could decrease cold harm and improve seedling index of pepper seedlings. Content of chlorophylls, soluble sugar and proline and POD activity in leaves from inducer treatment group were higher than that from CK, which characterized by ascending at first and descending at last. The maximum value of chlorophylls and soluble sugar content in inducer treatment group increased 25.93% and 61.54% respectively compared to CK, and the maximum value of proline content and POD activity increased 1.93 and 2.34 times respectively than CK. But the relative leakage of electrolytes and MDA content in inducer treatment group were lower than that of CK，which also characterized by ascending at first and descending at last. The minimum value of chlorophylls and soluble sugar content in inducer treatment group decreased 33.44% and 79.31% respectively compared to CK. Of the three inducers, salicylic acid showed the best cold resistance effect followed by betaine and paclobutrazol. And for salicylic acid, 7.5 mmol/L was the optimum concentration.