Full-length CDS of KAP6.1 gene was obtained by sequence assembly with 2 specific primers. Then the sequences in 6 rabbit populations were scanned by PCR-SSCP. The results showed that 3 genotypes and 2 alleles were found at KAP6.1-1 loci, as well as the A 755 G mutation which was located at the enhancer region, while no mutation was found at KAP6.1-2 loci. The distribution of these genotypes in all populations was consistent with the Hardy-Weinberg equilibrium and presented either intermediate level or low level of diversity; and its difference was either significant(P<0.05) or very significantly (P < 0.01) from one another populations except between Fujian black rabbit and Jiuyishan rabbit, chinchilla and white rex rabbit, wan-line and su-line angora rabbit (P>0.05).