This paper is an attempt to study the method of preparing the L-Tyr imprinted polymer by bulk polymerization, and investigated the property of the polymer. Based on the studying of the type and amount of functional monomer, crosslinking agent and the solvent and temperature which affected the preparation of molecularly imprinted polymer, further study was made to optimize the reaction conditions by doing orthogonal experiments of four factors in three levels. The structure of the polymer was analysed by fourier infrared spectrum; The selective adsorption of L-tyr was found by analysing polymers and extraction efficiency and adsorption capacity by fluorescence spectrometry. The results showed that in chloroform solvents, MAA was functional monomer; EDMA was crosslinker and AIBN was initiator. The molecular imprinted polymer which can selectively adsorb the L-Tyr could be obtained after being heated to 60 ℃ in water bath, extracting eluting solven according to the molar ratio：10∶40∶200∶1.5.