The structure, expression and function of pig TDRP1 gene was predicted with bioinformatics and comparative genomics methods. Results showed that：(1) The electronically cloned pig TDRP1 cDNA was 776 bp including a 119 bp 5′UTR, a 96 bp 3′UTR and an open reading frame (ORF) of 561 bp, which encoded a 187 amino acid peptide. (2) The CDS of pig TDRP1 had 85%, 76.1% and 77.1% with that of human, mouse and rat, respectively. The translated pig TDRP1 precursor had identities of 82.5%, 71.1% of identity homologous and 70.1% of identity homologous with its counterparts of human, mouse and rat, respectively. (3) The predicted molecular weight of pig TDRP1 was 20 489.8 Daltons without signal peptide. The pig TDRP1 might be a soluble and non-secretive protein, and probably forms four kinds of secondary structure. (4) Based on cDNA and EST library, the pig TDRP1 gene expresses at least in ovary and thyroid gland tissues.