Oryza officinalis conserved a lot of significant characters and specific genes. Although some excellent genes in O. officinalis were difficult to be transferred into cultivated rice by way of common breeding, they might be carried out through BIBAC (binary bacterial artificial chromosome) vector in the form of macro fragments. The genomic BIBAC library of O. officinalis. was constructed with the vector of BIBAC2 in this research. This library consisted of 53 760 individual clones stored in one hundred forty 384-well plates with an average DNA insert size of 76 kb within the scope of 25200 kb, equivalent to 5.86 times of genome.