Useing a 47 d fetus of Banna minipig inbred line as materials, fetal fibroblast cells were dispersed in vitro with trypsinnization and then subcultured. Observations on morphology, determination of viability, drawing of dynamic growth, and analysis of karyotype were carried out. The results showed that the cell was morphologically consistent with fibroblasts, cell viability was 98.06％ before freezing and 92.12％ after thawing, the growth curves was sigmoidal with population doubling time(PDT)of cells was approximately 36 h, karyotyping indicated a total chromosomes number 2n=38, sex chromosomes were XY, and the karyotype is normal afrer cultured up to passage 14 in vitro.