The BALB/c mice were immunized with DHV of allantoid liquid in duck embryo which purified by freezing and melting repeatedly, dealed with by chloroform , concentrated by PEG and overspeed membrane filtrating . Eight hybridoma cell lines were obtained after detecting and 3 sub-cloning by I-ELISA.They were named DHV-1、DHV-6、DHV-7、DHV-8、DHV-9、DHV-10、DHV-11 and DHV-12。The ascites titres of these MAbs were between 1:2000 and 1:512000. The ascites had no cross reaction with other antigen and the normal allantoid liquid in duck embryo. MAbs belonged to IgG withκchain. neutralization test and young duck pretection test indicated that neutralization characters of DHV-6、DHV-7、DHV-9、DHV-10 are better.