Homologous repeat region 2 (hr2) of Ectropis obliqua nucleopolyhedrovirus (EcobNPV) isolated from Hubei and Anhui strains were amplified by PCR. The results showed that only one 1.8 kb DNA fragment was amplified from EcobNPV-HB strains and two DNA fragments was amplified from EcobNPV-AH strains, which suggested that EcobNPV-HB strains was an independent strains different from EcobNPV-AH strains. hr2 of Ectropis obliqua nucleopolyhedrovirus isolated from Hubei strains was cloned into pMD18-T vector, sequenced and analyzed. The hr2 of EcobNPV-HB strains was composed of 1 761 nucleotides and coded 540 amino acids with a G+C content of 21.7%, comprised of perfect palindromes. Extra 362 nucleotides were observed compared with hr2 of EcobNPV-AH strains in GenBank.