In order to its degradation characteristics by use of GC, the relationship between micro-organisms, organic matter, light, initial concentration of florfenicol, and florfenicol in chicken feces were investigated, the degradation of florfenicol in non-sterilized chicken feces without organic matter removing was taken as the controlled group by culturing it in incubator with constant temperature at 20 ℃ under the dark.The result indicated that chicken feces was prevented from light under 20 ℃ condition, the half-life of florfenicol was 1.9 d, the degradation kinetic equation was Ct=7.81×e－0.369 9t, the degradation rate reached 90% within 11 d. Compared with the controled group, the degradation rate of florfenicol in chicken manure decreased markedly under the handling of sterilization and removal of organic matter. The half-lives grew up to 6.4 d and 7.2 d. The differences between the Initial concentration of 5 mg/kg and 10 mg/kg were not remarkable, and the half-life was 1.8 d and 1.9 d respectively, while when the Initial concentration rise to 20 mg/kg, the half-life extend to 5.8 d. Under either the condition of illumination or light prevention, the half-life, degradation kinetic equation and constant of degradation rate constant of florfenicol displayed no differences.