The complete genome of three subgroups(1A、1B、1C) Porcine circovirus 2 (PCV-2)was amplified by polymerase chain reaction(PCR)and cloned directly into plasmid pMD-18T, and recombinant plasmid carrying the complete genome was constructed，designated RP8（1A）、P0（1B）、P4-1（1C） respectively. The three entire genomes of PCV-2 were purified and recycled from recombinant pMD-18T with the digestion of SacⅡ enzyme and then circular genomic DNA were generated by self-ligating with T4 DNA ligase in vitro, resulting in three circular virus genome. The PCV1-free PK-15 cells were transfected with the PCV-2 circular genome using Lipofectin Reagent. At the fifth passages, PCV-2 virus and specific antigens were detected by PCR and indirect immunofluorescent assay(IFA).Mice were inoculated the cell cultures which were passed 5 times, the blood samples were collected 5 days post inoculation, the PCV-2 genomes were confirmed by PCR. The results indicate that the cloned circular PCV-2 genomic DNA is infectious both in vitro and in vivo, which provide good foundation for further study of the relationship between different genotype PCV-2 strains and their virulence.