We have cloned Nicotiana tabacum WS38 ABP1 cDNA coding sequence by RT-PCR with the primers that ware designed according to reported Nicotiana tabacum ABP1 amino acid sequence in GenBank. Sequencing shows that the length of the cDNA is 564 bp. Information analysis indicated that the open reading frame can encodes 188 amino acids polypeptide with the 98.4% homology with the reported ABP1. It demonstrated that the ABP1 in Nicotiana tabacum is varied in different cultivars and there are some single nucleic acid polymorphasim with WS38 and T85.