Based on the study of verification test and via orthogonal experiment in extracting Poria cocos polysaccharides of different factors including enzymolysis concentration, enzymolysis time, enzymolysis temperature and enzymolysis pH value, using resource-rich, high-pachyman Poria cocos as the raw material, the best technical parameter on the extraction of Poria cocos polysaccharides by the plant multienzyme SPE-002 was optimized and screened. The best parameters was:enzymolysis content 5.0% of the substrate, enzymolysis time 90 min, enzymolysis temperature 50 ℃, enzymolysis pH 5.0 .Compared with the traditional extraction ratio 1.6%, the optimized extraction yield reached 11.78% by use of the plant multienzyme SPE-002.