The young leaves and stems of Camptotheca acuminata were used as the materials to study the effects of basic media and the phytohormone combination on callus induction and subculture of the callus and screen out the best cultural formula for cell suspension culture system of Camptotheca acuminata. The results showed that most suitable medium for callus induction was SH with 2.0 mg/L NAA and 0.5 mg/L 6-BA, and the callus inducing rate was 92%. MS medium with 2.0 mg/L 6-BA and 0.5 mg/L NAA was found to be the best subculture medium formula, and the callus induced under these conditions and it was suitable for cell suspension culture.