The cDNA of DREB transcription factor was obtained from Chaling wild rice under low temperature by method of RT-PCR. The sequence analysis indicated: The clone consisted of 958 bp, coding 314 amino acids; The homlogous of sequence from Oryza sativa was 98%, 93% of which came from Triticum aestivum CRT/DREB4; The homlogous from Hordeum vulgare protein CBF2A and CBFIVc-14.1 was 93% and 92%; The deduced primary structure of DREB contained a single AP2 domain, showing the typical characteristics of DREB gene family. A plant expression vector which is named pWM101DREB of the DREB gene was successfullyconstructed, which laid the foundations for future transgenic research on DREB gene function.