In this study, we ligated the BCCodY gene of Bacillus choshinensis strain 2-Q-9 to vector pCHF3, and then transformed the recombination vector pCHF3::BCCodY into tobacco variety K326 by Agrobacterium-mediated approach. Transgenic K326 plants were identified by polymerase chain reaction (PCR). Reverse transcription (RT)-PCR was performed to analyze expression of BCCodY gene in transgenic plants. The results illustrated that BCCodY gene can be transcribed driven by CaMV 35s promoter. To characterize the biological function of BCCodY in transgenic K326 plants, we inoculated transgenic plants with Rolstonia solanacearum strain RS2-1 by injection. The results showed, the colony number of RS2-1 isolated from transgenic K326 plants was fewer than wild plants in 7th day after inoculation, but the difference of colony number of transgenic and wild plant was gradually disappear after 9 days. It can be deduced that the BCCodY gene maybe performed antagonistic ability against R. Solanacearum in transgenic K326 plants.