Taking the flue–cured tobacco variety Jiyan No.9 as material, the combined effects of varied Nitrogen volume and apical regulator on chromoplast pigment and the content of its degradation products in flue-cured tobacco leaf were determined. Results suggested that both of the treatments, namely different Nitrogen volume and apical regulator, significantly affected chromoplast pigment and the content of its degradation products in post-cured tobacco leaf, additionally, the combined effects of the treatments mentioned above on carotenoid reached to extremely significant level. Findings were :(Ⅰ) The content of chlorophyll, carotenoid and their decomposition products in tobacco leaf of high and medium Nitrogen rate treatments were relatively higher and could meet the demand for nitrogen element during growth and developmentf tobacco. (Ⅱ) Apical regulator probably enhanced the physiological activity of tobacco plant and promoted the degradation and conversion of chromoplast pigment.The content of neophytadiene and the degradation products of carotenoid in treatment of apical regulator B, particularly, evidently higher than that of other ones. Comparison of different treatments, as existing interactive action between two factors, the treatment of T8, namely medium Nitrogen volume coupled with apical regulator B, could achieve the highest content of chromoplast pigment degradation products in post-cured tobacco and also relatively better tobacco character.