To understand the genetic mechanism of exserted stigma, a mapping population of 190 F2 plants derived from the cross between a japonica variety-Nuo 5 and an indica variety-YouⅠB with higher percentage of exserted stigma (PES) was constructed to map quantitative trait loci (QTL) for PES. Genetic linkage map was constructed using 92 SSR markers throughout the whole genome, and PES was investigated in Changsha. As a result, three QTLS controlling ratio of stigma exsertion for qPES-2, qPES-5, qSPES-8,detected and located respectively at the region of RM1285-RM12595 on chromosome 2 and RM17952-RM18114 on chromosome 5 and RM8020-RM7080 on chromosome 8, the QTL of LOD peak 3.54, 4.79, 3.85, respectively, of the phenotypic variation were 10.1%, 11.1%, 9.0%,and all their positive alleles were from YouⅠB. Two QTLs might be identified in previous studies, and the QTL on chromosome 5 might be a new one which was never reported.