Abstract：The sugar contents of 6995 flue-cured tobacco samples including B2F，C3F and X2F leaves of Hongda, K326, Yunyan85 and Yunyan87 from Yunnan Province were analyzed with continuous flow analyzer. The results showed that Yunnan tobacco leaves characterized rich sugar. Total sugar contents range of upper，middle and lower leaves were 22%~36%，28%~40% and 26%~38% with means of 29%，33% and 31% respectively，and reducing sugar contents ranges were 20%~30%、24%~34% and 22%~32% with means of 25%，28% and 27% respectively，and TS-RS were 3.8%，4.5% and 4.5% respectively. There were significant difference of sugar contents among planting areas，annual means and varieties. The sugar contents of leaves from west was higher，followed by centre，east and southwest，and northeast and southeast of Yunnan were lower. The sugar contents of Hongda and K326 were higher than that of Yunyan85 and Yunyan87. TS-RS of Hongda is the lowest. Keywords Yunnan tobacco planting area; Flue-cured tobacco; Sugar