Taking flue-cured tobacco varieties JY-02 as experimental material，effects of 3 different antivirus agent on disease resistance of tobacco plant and quality of Flue-cured tobacco were studied in YanBian city，Jilin province，in 2007. Results indicated that oumaisi1500 times had the best controlling effect on CMV，the effect still kept at 82.14%; various periods of PVY，yichuanling100 times had the best controlling effect on PVY，the average effect still kept at 59.24%，the natural incidence was the lightest in field. All different antivirus agent treatments had some role in promoting the quality of tobacco leaves. oumaisi1500 times could coordinate the proportion of the chemical component，the yield，the average price，the proportion of superior tobacco leaves had different degrees of improvement，and had the optimal filling value、thickness of leaves、tensile strength、stem concent and density of leaf fabric；The content of aroma components such as the contents of carotenoid、phenylalanine and cembratriendiol were prompted，the total amount of flavor matter and different types of aroma substances increase significantly. In summary，yichuanling100 times could effectively control PVY，but its effects on improving quality of tobacco was poor；while the best controlling effects on CMV and flue-cured tobacco leaf quality by oubeisi1500 times were better than any other treatment，so its use was worth being promoted in YanBian area.