The chicken GH和POU1F1 gene was selected as candidate genes，PCR-SSCP method was used to identify the polymorphism sites in the exon4 region of GH gene and in the exon3 region of POU1F1 gene.Association between the founded polymorphism sites as well as pyramiding genotypes and egg production traits were tested by least square analysis. The results showed the founded polymorphism sites （A5331T）GH gene exon4 had significant correlation with the egg number at 72 weeks(P<0.05). The genotype AA additive effects for GH genes were 3.80. The founded polymorphism sites（A5331T） POU1F1 gene exon3 had significant correlation with 72 weeks of age egg production. The genotype CC additive effects for POU1F1 genes were 3.57.The pyramiding genotype AACC had higher 72 weeks of age egg production than that of other pyramiding genotypes(P<0.05). The interaction effect was not significant (P>0.05)。The generation F1 egg production as their motherhood.