A residue analytical method was developed for the determination of difenoconazole in Panax notogineseng. Difenoconazole residues were extracted by ultrasonic from Panax notogineseng with acetone, purified by a column of aluminum and a column of silica gel in turn , then detected by GC-ECD. Its limit of detection was 0.004 ng, its minimum detectable concentration of difenoconazole in the samples was 0.02 mg/kg. Its average recovery of ranged from 80.6% to 95.0% and its relative standard deviation ranged from 1.6% to 11.9% when the samples are spiked difenoconazole at 0.02~0.5 mg/kg(n=5) level. These results showed that in sensitivity, precision and accuracy, the method met the demand of pesticide residue analysis. Market random tests showed that difenoconazole residues in Panax notogineseng between 0.02 to 0.6 mg / kg, the detection rate was 47%, the detection rate and residue levels from high to low were Panax notoginseng flowers, Panax notoginseng fibril roots, Panax notoginseng in turn.