Using Boaibayuehuang Diospyros kaki dormant buds, sprout buds and shoot tips as explants, effect of sampling time, disinfection time, disinfectants and plant growth regulators on culture of their organizations. The results showed that highest germination ratio of dormant buds, sprout buds and shoot tip reached 70%, 50%, 30% respectively, that of dormant bud (dormant period explants) is the highest, and its proliferation ability is stronger; best sampling period is around December 6, using dormant buds, sprout buds as explants could establish the beginning of culture, while the beginning of shoot tip culture is not easy to be established. Using 0.1%HgCl2 to disinfect 5 min will be best to explant at its earlier growing period, its pollution rate, the browning rate is relatively lower, while its survival rate is the highest; it would be better to use 0.1% HgCl2 to disinfect explants for 10 min, although its pollution rate is higher, but its browning rate is the lowest, the survival rate is the highest; Comparison of disinfectants (NaClO and HgCl2) indicated the optimal level of disinfectant to explant in the dormant period is 0.1%HgCl2, the disinfection time is 15min,the survival rate is higher, reaching 70.2%. The most suitable culture medium for Boaibayuehuang Diospyros kaki is 1/2 MS+1 mg/L ZT +0.1 mg/L IAA +4.0 mg/L 6-BA.