Ranunculus asisticus seeds were treated with (－1±0.2),(2±0.2),(5±0.2) ℃ for 15, 30, 45 d, its effects on seeds germination were studied. Rresults showed that: the pre-chilling could significantly increase the seed germination. Compared to CK, the best treatment was (-1±0.2) ℃ for 30 d(P< 0.01), in which the seeds increased by 37.70% in its germination energy and by 21.60% at its germination rate and by 0.36 in its vigor index.