红花檵木的AFLP分析及分类
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湖南省自然科学基金项目(04JJ3024);湖南省教育厅省高等学校产业化培育项目(07CY001)


Classification of Loropetalum chinense var. rubrum based on AFLP analysis
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    摘要:

    用荧光AFLP技术,对所收集的22个红花檵木材料和变异类型进行分类与亲缘关系研究.从64对引物组合中筛选出8对引物组合进行扩增,共扩增出 1 226条带,多态条带1 085条,多态性比率为88.5%.冬艳紫红、大圆叶双面红的多态性比率最高,达100%,其同源性最低.基于AFLP分析的UPGMA聚类分析得到,供试材料的遗传相似系数为0.744 2~0.920 7.以0.817 1的相似系数为阈值, 所有供试材料被分为6大组群,第一组群内以0.848 4为阈值,又可分为 4组.通过AFLP标记发现冬艳红5个类型中冬艳玫红、冬艳亮红、冬艳卷瓣玫聚类在一起,冬艳紫红、冬艳卷瓣红与其他类型存在的遗传差异通过分子标记可以鉴定出来.通过标记发现大圆叶双面红并没有与大叶玫红、大叶红、大叶卷瓣红、大红伏划分在双面红类型中,其外部形态相似,但遗传距离有较大差异.与形态标记相比,基于AFLP标记的红花檵木分类体系更能反映红花檵木品种间的亲缘关系.

    Abstract:

    22 Loropetalum chinense var. rubrum were subjected to fluorescent AFLP(amplified fragment length polymorphism) analysis and its classification of variation types and kinship were investigated. Eight primer combinations selected from 64 primer combinations produced a total of 1 226 strips, of which 1 085 were polymorphic with a polymorphism percentage of 88.5%. The polymorphism percentage of Dongyanzihong, Dayuanyeshuanmianhong are 100%, their homology were the lowest. An UPGMA dendrogram based on the AFLP alleles showed that genetic similarity coefficients among Loropetalum chinense species was between 0.744 8 and 0.920 7. All the materials were divided into 6 groups with the threshold of 0.817 1 of similarity coefficient. At the same time, the first group was divided into 4 subgroups with the threshold of 0.848 4 of similarity coefficient. The AFLP result showed that Dongyanhong including 5 types, only Dongyanmeihong, Dongyanlianghong, Dongyanjuanbanmei was grouped together as the morphologic markers classified, the genetic differences of Dongyanzihong and Dongyanjuanbanhong could be appraised by the molecular marker from the other 3 types. The AFLP result also showed that Dayuanyeshuanmianhong was not grouped together with Dayemeihong, Dayehong, Dayejuanbanhong and Dayehong as the morphologic markers classified. Thus, tyey have morphological similarity but presented great differences in the genetic distance. Compared with morphologic markers, the classification system based on AFLP markers could reflect relationship Loropetalum chinense var. rubrum cultivars more efficiently.

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李达.红花檵木的AFLP分析及分类[J].湖南农业大学学报:自然科学版,2010,36(2):169-176.

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  • 收稿日期:2009-09-26
  • 最后修改日期:2009-10-16
  • 录用日期:2010-03-10
  • 在线发布日期: 2010-04-03
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