A quick and efficient method for simultaneous determination of nicotine, nornicotine, myosmine, anabasine and anatabine by GC-MS was developed.The optimum conditions of ultrasonic extraction and organic solvent extraction were radicated by the orthogonal design and volume of the extraction solvent was optimized. Regression function, recovery and precision of the method were reviewed. The results show that the linear ranges of nicotine, nornicotine, myosmine and anabasine were 20─600, 30─300, 3.2─12 and 16─60 mg/L, respectively. and the detection limits were 0.11, 0.09, 0.02 and 0.04 mg/kg, respectively. The recoveries were in the range of 88.5%─105.4% and the method precisions were in the range of 2.4%─9.2%. The test shows that the method comes on with high sensitivity, good precision good reproductibilty and convenient operation.The method has solved the problerm that alkaloids extraction was imperfect and the myosmine detection was ineffective.