The nucleic acid and protein sequences on NSs, N and S RNA full-length sequences of Tospovirus genus were downloaded from NCBI database, and they were aligned by bio-informatics software DNAstar, DNAMAN and NPSA. It was demonstrated that homology between CCSV and TZSV was 85.8% on homologous phylogenetic tree on NSs protein sequences, homology between IYSV and TYRV reached 90%, homology between MYSV and PSMV was up to 97.5%. There are 7 groups of high homology with analysing the N protein sequences. Tospovirus is rich in non-polar amino acids but polar-acid amino acid is the lowest. At the same time, by alignment TSWV strains between resistance-breaking strain and wild strains on NSs, N nucleic acid and protein sequences, amino acids and the protein secondary structure with no significant differences found.