鹰嘴豆EST资源中SSR的信息分析
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家科技支撑计划项目(2006BAD09A04,2006BAD09A08,2007BAC15B06);国家自然科学基金项目(30860152);国家博士后科学基金项目(20080431107 );高等学校学科创新引智计划项目(教育部);科技支疆项目(200991254)


Information analysis of EST resource of chickpea
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    从NCBI公共数据库下载获得27 904条鹰嘴豆表达序列标签EST,去除其中低质量的和冗余序列后得到全长为6 198 092 bp的11 224 条无冗余EST.利用SSRIT(Simple sequence repeat identification tool)软件共在这些序列中发掘出982个SSR,它们分布于865条EST中,出现频率为8.69%,平均长度为15.58 bp,平均距离为6.31 kb.在鹰嘴豆EST-SSR中,二核苷酸重复是最主要的重复类型(66.90%),其次是三核苷酸重复类型(30.75%),出现最多的重复基元类型是TA/AT(24.95%).

    Abstract:

    27 904 ESTs of chickpea (Cicer arietinum L.) were downloaded from the database of NCBI and some redundant or low quality sequences were removed. Finally, 11 224 non-redundant ESTs with total length of 6 198 092 bp were obtained. SSRIT (Simple sequence repeat identification tool), a web tool for SSR hunting, was used to analyze the sequences. The results showed that a total of 982 SSRs distributed in 865 ESTs were detected, with the frequency for the non-redundant ESTs being 8.69%. The average length and distribution distance of the EST-SSRs were about 15.58 bp and 6.31 kb. Among the different SSRs ranging from 2 to 7 bp, dinucleotide repeats were most abundant (66.90%), followed by trinucleotide repeats (30.75%). Among 83 identified motif types, TA/AT had the highest EST frequency (24.95%). The results would contribute to the development of EST-SSR markers in chickpea.

    参考文献
    相似文献
    引证文献
引用本文

王显生,贾钰莹,顾汉艳,何小玲,张桦,张巨松,石书兵,李建贵,麻浩.鹰嘴豆EST资源中SSR的信息分析[J].湖南农业大学学报:自然科学版,2010,36(2):137-141.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2009-09-01
  • 最后修改日期:2009-12-14
  • 录用日期:2010-03-04
  • 在线发布日期: 2010-04-04
  • 出版日期:
文章二维码