Indica rice cultivars of Han116 and Xiangzaoxian32 were used as experimental materials with different concentration (0%, 5%, 10%, 15%, 20%, 25%)PEG6000, the relationship between change of polyamine contents and drought-resistance in rice seedlings under water stress was investigated. The results showed the contents of Put, Spd and Spm significantly increased under water stress, Han116 increased more markedly than Xiangzaoxian32, which was a positive correlation between Put, Spd and Spm contents and root/shoot. Through regression analysis, Put, Spd and Spm were respectively led into by y1=1.248E-04x1+0.272, y1=1.421E-05x2+0.240, y2=－1.55E-05x2+4.922E-04x3+0.126, y2=4.679E-04x3+3.923E-02 equations(x1, x2, x3 respectively mean Put, Spd and Spm ,y1 , y2 respectively mean shoot and root dry weight ).