Conditions in deep submerged fermenttation of Armillarialuteo viren was studied for exploiting Armillarialuteoviren resources by one-factor experiment. The results showed that the optimum carbon sources was sucrose and the optimum nitrogen sources was sucrose beef extract. The optimum medium by the orthogonal test was sucrose 4%, beef extract 0.1% , KH2PO4 0.10% and MgSO4 0.10 %. The fermentation conditions were as follows: seed age 4 d , inoculation rate 15% ,culture time 8 d. Under these conditions, myceliun biomass could reach 1.137 mg/mL.