In order to find linked RAPD markers for the glucosinolate trait in Brassica napus, 967H and 967L were used as materials，which kept similar characteristics in quality and other agronomic traits except for the glucosinolate trait, to study RAPD markers of the parents, their F1 and F2 plants. The results showed that 6 RAPD primers from 482 RAPD primers were detected polymorphism between parents, and one RAPD primer(S268) from the 6 RAPD primers were detected polymorphism between two parents and two DNA bulks. The primer S268 could only display three kinds of different marks. Variant analysis with a single mark indicated that the low glucosinolate traits of 29.51％ were contributed by the S268600 mark.