吴磊,覃媛钰,谭光辉,李杰章,张依裕.长顺绿壳蛋鸡OC–116基因变异对蛋壳品质的影响[J].湖南农业大学学报:自然科学版,2019,45(6):.
长顺绿壳蛋鸡OC–116基因变异对蛋壳品质的影响
  
DOI:
中文关键词:  长顺绿壳蛋鸡  OC–116基因  单核苷酸多态性  蛋壳品质
英文关键词:Changshun blue-eggshell chicken  OC-116 gene  single nucleotide polymorphism  eggshell quality
基金项目:贵州省科技合作计划项目(黔科合LH字〔2016〕7454号)
作者单位
吴磊,覃媛钰,谭光辉,李杰章,张依裕 1.贵州大学动物科学学院贵州 贵阳 5500252.高原山地动物遗传育种与繁殖教育部重点实验室贵州 贵阳 550025 
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中文摘要:
      以长顺绿壳蛋鸡为试验对象,采用PCR产物直接测序法,对OC–116基因g.45670562—g.45670994和g.45669431—g.45670163区域进行单核苷酸多态性筛查;运用一般线性模型,分析多态位点对长顺绿壳蛋鸡蛋壳品质的遗传效应。结果显示:OC–116基因存在4个SNP位点,分别为位于第2外显子上的g.45668081 G>T、第3外显子上的g.45669143 T>C、第3内含子上的g.45669061 A>G和g.45669101 T>A,g.45668081 G>T和g.45669143 T>C为同义突变,4个SNP位点均表现为中度多态;卡方(χ2)检验表明,4个SNP位点的基因型分布均未偏离Hardy–Weinberg平衡(P>0.05);关联性分析结果表明,g.45669061 A>G位点的AA和AG基因型的蛋壳厚度显著高于GG基因型的(P<0.05),g.45669143 T>C位点的TT和CT基因型的蛋壳强度和蛋壳厚度显著高于CC基因型的(P<0.05),揭示g.45669061 A>G和g.45669143 T>C位点可作为蛋壳性状选择的标记性位点。
英文摘要:
      Using Changshun blue-eggshell chicken as the test object, single-nucleotide polymorphisms were screened in g.45670562-g.45670994 and g.45669431-g.45670163 of OC-116 gene by direct sequencing of PCR products; the genetic effect of polymorphic loci on shell quality of Changshun blue-eggshell chicken was analyzed using a general linear model. The results show that there are 4 SNP loci in the OC-116 gene, which are g.45668081 G>T on exon 2, g.45669143 T>C on exon 3, and intron 3 g.45669061 A>G and g.45669101 T>A. The g.45668081 G>T and g.45669143 T>C are synonymous mutations. The all four SNP loci show moderate polymorphism. The result of Chi square(χ2) test showed that the genotype distribution of the four SNP loci localized in the range of Hardy-Weinberg equilibrium(P>0.05). And, the correlation analysis showed that the g45669061 A>G loci had eggshells of AA and AG genotypes with the thickness significantly higher than that of GG genotype(P<0.05) and the g.45669143 T>C locus had eggshells of TT and CT genotypes owing significantly higher in egg shell strength and egg shell thickness than CC genotype(P<0.05). This results reveal the g.45669061 A>G and g.45669143 T>C sites can be used as marker sites for eggshell trait selection.
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