李启武,金红玉,舒倩,干兴利,杨海君,邢宏霖.固定化混合菌高效降解残杀威的条件优化[J].湖南农业大学学报:自然科学版,2019,45(6):.
固定化混合菌高效降解残杀威的条件优化
  
DOI:
中文关键词:  残杀威  不动杆菌  混合菌  固定化微球  降解  条件优化
英文关键词:propoxur  mixed bacteria  immobilization  degradation  conditions optimization
基金项目:湖南省自然科学基金项目(2018JJ5015);长沙市科学技术局项目(kq1801021)
作者单位
李启武,金红玉,舒倩,干兴利,杨海君,邢宏霖 1.国家环境保护重金属污染监测重点实验室湖南 长沙 4100142.湖南农业大学植物保护学院湖南 长沙 410128 
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中文摘要:
      从海利(常德)农药化工有限公司废水处理池污水中分离得到2株耐盐残杀威降解细菌CS1和CS2,经形态特征、生理生化鉴定及16S rDNA序列分析,2菌株均属于不动杆菌(Acinetobacter sp.)。以处于对数生长期的CS1和CS2菌株菌悬液分别按照体积比为1∶1、1∶1.5、1∶2、1∶2.5、1∶3、1.5∶1、2∶1、2.5∶1、3∶1复配成混合菌剂,结果CS1和CS2菌悬液体积比为1.5∶1(M6)时,对残杀威的降解率最高,达到72.68%。采用海藻酸钠(SA)–活性炭、聚乙二醇(PVA)–活性炭及PVA–SA–活性炭3种组合材料对M6进行包埋,PVA–SA–活性炭固定化M6对残杀威的降解率为73.22%。采用正交试验对影响残杀威降解效率的4个主要因素(残杀威质量浓度、培养基盐度、温度和pH值)进行优化,M6降解残杀威的最佳组合为残杀威质量浓度200 mg/L、盐度3%、pH 6.5、温度30 ℃,对残杀威的降解率达77.34 %;对固定化混合菌M6降解残杀威的影响大小依次为温度、残杀威质量浓度、盐度、pH值。
英文摘要:
      Two strains of salt-resistant propoxur-degrading bacteria CS1 and CS2 were isolated from the waste water treatment tank of Hali (Changde) Pesticide Chemical Co., ltd., and identified as Acinetobacter sp. through morphological characteristics, physiological and biochemical identification and 16S rDNA sequence analysis. The suspensions of CS1 and CS2 strains in the logarithmic growth stage were compounded into mixed bacterial agents according to the volume ratio of 1∶1, 1∶1.5, 1∶3, 1.5∶1, 2∶1, 2.5∶1 and 3∶1, respectively. Results showed that when the liquid volume ratio of CS1 and CS2 suspensions was 1.5:1(M6), the degradation rate of propoxur was the highest, reaching 72.68%. M6 was embedded with sodium alginate (SA)-activated carbon, polyethylene glycol (PVA)-activated carbon and PVA-SA-activated carbon. A degradation rate of 73.22% was observed when the strains were embedded in PVA-SA-activated carbon. The orthogonal test was used to optimize the 4 main factors that affect the degradation efficiency of propoxur (propoxur mass concentration, medium salinity, temperature and pH). The optimal combination of the 4 factors for degradation of propoxur by M6 include propoxur mass concentration of 200 mg/L, salinity of 3%, pH 6.5 and temperature of 30 ℃, and the degradation rate of propoxur was up to 77.34%. Degradation of propoxur by immobilized mixed bacteria M6 was effected mostly by temperature, followed by propoxur mass concentration, salinity and pH.
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