李海娟.Thermus thermophilus HB27质粒分离基因位点同系物parATth的功能研究[J].湖南农业大学学报:自然科学版,2019,45(6):.
Thermus thermophilus HB27质粒分离基因位点同系物parATth的功能研究
  
DOI:
中文关键词:  噬热栖热菌  质粒分离基因位点parABS  染色体  亚细胞定位
英文关键词:Thermus thermophilus  partitioning locus parABS  chromosome  subcelluar localization
基金项目:国家自然科学基金项目(31700059);陕西省自然科学基础研究计划项目(2018JQ3037);陕西省高校科协青年人才托举计划项目(20180210)
作者单位
李海娟 西安文理学院生物与环境工程学院陕西 西安 710065 
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中文摘要:
      通过敲除噬热栖热菌(Thermus thermophilus)HB27的parATth基因,获取parATth基因敲除突变体,并从染色体DNA的复制及子细胞分离等方面分析突变体的表现型,鉴定parATth的功能。结果显示:在parATth突变体中,未出现明显的细胞生长与分裂受阻现象,细胞形态也未发生变化;与野生株相比,突变株染色体DNA的拷贝数未发生变化,大区段染色体DNA的子细胞分离也未受阻,但染色体的复制起始位点(oriC)区域的亚细胞定位受到影响,其定位于细胞任意位置。综合分析,T. thermophilus HB27的parATth基因在大区段染色体DNA的子细胞分离过程中没有起关键作用,但其可能参与染色体复制起始位点区域的子细胞定位与分离。
英文摘要:
      In this study, the parATth gene mutant, ΔparATth, was generated by deleting the gene in Thermus thermophilus HB27 and were subjected to phenotype analysis with respect to chromosome replication and segregation. The results showed that in the ΔparATth mutant, the cell growth, cell division, cell morphology were not apparently affected. And, the chromosome copy number was not changed in the mutant and the chromosomal bulk DNA segregation was not impeded either. However, the subcelluar localization of the chromosomal origin (oriC) was randomly localized in the mutant cells instead of the polar localization pattern in the wild type cells. Therefore, in T. thermophilus HB27, the parATth gene is not critical for the chromosomal bulk DNA segregation, however, it might be involved in the chromosomal origin region localization and segregation.
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