尹坚,王振华,刘天波,李基光,陈武,肖云花,李迅,唐前君.烟草黑胫病拮抗细菌菌群的筛选[J].湖南农业大学学报:自然科学版,2019,45(6):.
烟草黑胫病拮抗细菌菌群的筛选
  
DOI:
中文关键词:  烟草  烟草黑胫病  高通量测序  拮抗细菌菌群  筛选
英文关键词:tobacco  tobacco black shank  high-throughput sequencing  antagonistic bacterial flora  screening
基金项目:中国烟草总公司湖南省公司项目(17–20Aa02);湖南省教育厅开放基金项目(16K039)
作者单位
尹坚,王振华,刘天波,李基光,陈武,肖云花,李迅,唐前君 1.湖南农业大学植物保护学院湖南 长沙 4101282.植物病虫害生物学与防控湖南省重点实验室湖南 长沙 4101283.湖南省烟草公司张家界市公司湖南 张家界 4270004.湖南省农业科学院作物所湖南 长沙4101255.湖南农业大学生物科技学院湖南 长沙 4101286.爱荷华州立大学植物病理与微生物系美国 艾奥瓦州 50010 
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中文摘要:
      从湖南烟区烟草黑胫病重发田块采集根际土壤样品共900份,经富集培养后筛选出195个对烟草黑胫病菌有拮抗效果的细菌菌群。菌群Miseq高通量测序结果表明:不动杆菌属(Acinetobacter)和寡养单胞菌属(Stenotrophomonas)为拮抗细菌菌群共有的优势属。挑选5个抑菌效果较好菌群施入烟田土壤,B44R菌群对烟草黑胫病防效达51.56%。将这5个细菌菌群进行拮抗菌株分离筛选,获得的拮抗菌株分别属于芽孢杆菌属、不动杆菌属、寡养单胞菌属和克雷伯氏菌属。施入菌群后的土壤高通量测序结果显示这些拮抗细菌菌群可在土壤中定殖,其中不动杆菌属和芽孢杆菌属的种群数量显著增加。
英文摘要:
      A total of 900 rhizosphere soil samples were collected from the main tobacco producing areas in Hunan Province and 195 bacterial groups with antagonistic effect on tobacco black shank were screened out after enrichment and cultivation. The high-throughput sequencing results of Miseq showed that Acinetobacter and Stenotrophlomonas are the common dominant genera of antagonistic bacteria. Five bacteria groups with better bacteriostatic effect were selected and applied to the soil of tobacco field, the control effect of group B44R on tobacco black shank was 51.56%. Antagonistic strains in the 5 bacteria groups were screened and isolated, the obtained antagonistic strains were found to belong to 4 genera: Bacillus, Acinetobacter, Stenotrophomonas and Klebsiella. The results of soil high-throughput sequencing showed that after applied to soils, the antagonistic bacteria could colonize in the soil and the populations of Acinetobacter and Bacillus increased significantly.
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