吴彦庆,姜宇,赵大球,陶俊.芍药查尔酮异构酶基因CHI的表达特性分析[J].湖南农业大学学报:自然科学版,2017,43(4):.
芍药查尔酮异构酶基因CHI的表达特性分析
  
DOI:
中文关键词:  芍药  查尔酮异构酶基因CHI  花瓣  基因表达
英文关键词:Paeonia lactiflora  chalcone isomerase (CHI) gene  petal  gene expression
基金项目:国家自然科学基金(31372097,31400592);江苏省高校自然科学研究重大项目(13KJA210005)
作者单位
吴彦庆,姜宇,赵大球,陶俊 扬州大学 a.动物科学与技术学院b.园艺与植物保护学院江苏 扬州 225009 
摘要点击次数: 19
全文下载次数: 10
中文摘要:
      为了揭示查尔酮异构酶基因(chalcone isomerase gene,CHI)在芍药花瓣中的表达规律与特点,以不同芍药托桂型品种(‘金辉’ ‘彤云金焰’‘红楼锦菊’)4个不同发育时期(花蕾期、初开期、盛开期、衰败期)中的内、外花瓣为对象,用qPCR分别检测CHI基因的表达水平,分析其在不同芍药品种、不同发育时期以及内外花瓣之间的表达差异。结果显示:不同发育时期同一品种芍药花瓣组织CHI基因的表达量存在一定差异,从花蕾期到衰败期整体表现为上升趋势;不同品种同一发育时期‘彤云金焰’花瓣组织(内瓣和外瓣)CHI基因的表达水平明显高于‘金辉’和‘红楼锦菊’的;同一芍药品种在相同发育时期内瓣组织的CHI基因表达量均明显高于外瓣组织的。鉴于不同芍药品种、不同发育时期内外花瓣间颜色存在差异,推测CHI基因表达量可能与芍药花瓣颜色的形成有关。该结果可为深入研究芍药CHI功能并开展芍药花色遗传改良分子育种研究提供技术支撑。
英文摘要:
      Chalcone isomerase (CHI) is a key gene in regulating the formation of yellow petals. Inner and outer petals from different Paeonia lactiflora cultivars including Jinhui, Tongyunjinyan, Hongloujinju at four growing stages (flower–bud stage, initiating bloom stage, bloom stage, wither stage) were selected as materials to study expression pattern and characteristic of CHI gene. Expression difference of CHI gene in inner and outer petal at four growing stages among different Paeonia lactiflora cultivars were conducted using qPCR technology. The results showed that the expression level of CHI gene exhibited difference in petal tissues from the same cultivar and showed a rise trend from flower bud stage to wither stage; For different cultivars, the expression level of CHI gene in petal tissues of Tongyunjinyan was apparently higher than that of Jinhui and Tongyunjinyan at the same growing stages, furthermore, it was apparently higher in inner petals than that in outer petals for the same cultivars at same growing stages. Considering color difference in inner and outer petals at different growing stages and different Paeonia lactiflora cultivars, it was speculated that the expression level of CHI gene might be related to the color formation of petals in Paeonia lactiflora. The result could provide theoretical guidance for further research on the CHI function and genetic improvement to flower color of Paeonia lactiflora.
查看全文  查看/发表评论  下载PDF阅读器
关闭
Copyright © 2008 《湖南农业大学学报》自然科学版 地址:湖南 长沙市 芙蓉区 
邮编:410128 电话:0731-84638380 0731-84618035 0731-84617599 0731-84673730 E-mail: zkb4618035@hunau.net  京ICP备09084417
分享按钮