汤宾,李玉军,赵燕,黄丽华,黄妤,张学文.十字花科植物17个物种PIN3基因的系统进化分析[J].湖南农业大学学报:自然科学版,2017,43(4):.
十字花科植物17个物种PIN3基因的系统进化分析
  
DOI:
中文关键词:  十字花科  PIN3基因  系统进化
英文关键词:Brassicaceae  PIN3 gene  phyletic evolution
基金项目:湖南省教育厅高校重点实验室开放项目(15K060)
作者单位
汤宾,李玉军,赵燕,黄丽华,黄妤,张学文 湖南农业大学生物科学技术学院湖南 长沙 410128 
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中文摘要:
      为了探明十字花科植物不同物种PIN3基因之间的进化差异,运用Brassica Database数据库及十字花科基因数据库在线平台,对拟南芥(Arabidopsis thaliala)、红花荠菜(Capsella rubella)、芜青(Brassica rapa)、油菜(Brassica napus)、甘蓝(Brassica oleracea)等17个已完成基因组测序的十字花科物种的18个(油菜含有2个)PIN3同源性基因进行分析,并对其推导的编码蛋白(PIN3)进行了系统进化分析。结果表明,18个PIN3序列间的相似性为86.74%,PIN3推导蛋白之间的相似度为90.93%,蛋白等电点约为7.15~9.44,芜青PIN3等电点明显偏高。PIN3的氨基酸比对分析结果表明,在多个糖基化位点出现具有种属差异性和符合进化关系的氨基酸替换现象,盐芥属植物中条叶蓝芥(Thellugiella parvula)PIN3在更多的位点出现氨基酸的替换,这可能是导致十字花科不同种属PIN3蛋白的功能和活性出现差异的原因。
英文摘要:
      In order to explore the evolutionary differences between PIN3 genes in different species of Brassicaceae, the Brassica database and the Brassicaceae gene database were used. In this paper 18 PIN3 genes were identified by sequences blast in the database of 17 sequenced Brassicaceae species (there were two homologous PIN3 in Brassica napus). The phylogenetics of the genes and their predicted proteins (PIN3) were carried out by bioinformatics analysis. The results indicated that the similarity of 18 PIN3 gene sequences in the Brassicaceae species was 86.74%, while the proteins similarity was 90.93%.The PI of PIN3 protein was form 7.15 to 9.44, the PI of Brassica rapa was higher than those of others. The amino acid difference was found in several glycosylation sites of PIN3 protein with species diversity, which conformed to the amino acid substitution phenomenon and may result in difference function and activity of the protein in evolutionary relationships.
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