The burley tobacco variety of TN90LC with low content of tobacco–specific nitrosamines(TSNAs) and N'?nitrosonornicotine (NNN) was introduced from America. The results of experiment and evaluation indicated that the content of TSNAs, NNN and the nornicotine conversion of TN90LC were significantly lower than those of Eyan 1 (control variety). The average yield of TN90LC was 2 638.60 kg/hm2, and the percentage of the sum of first grade tobacco and middle grade tobacco reached 86.06%. The resistant results showed that TN90LC has moderate to normal resistance to black shank, and normal resistance to TMV, moderate resistance to root knot nematode disease. The appearance quality and sensory quality of TN90LC were both better than those of the control, the content of main chemical components was appropriate, and the harmony of chemical components was also better. By comprehensive analysis, the TN90LC was an excellent burley tobacco variety which is compatible in several important traits, including less–harmful, fine quality, proper yield and enough resistibility to main diseases.