The swine testicular(ST) cells infected with transmissible gastroenteritis virus (TGEV) were treated with alkaloids of Coptis chinensis(ACC) with mass concentrations of 100, 50 and 25 μg/mL respectively. MTT assay was used to detect the cell viability, the griess method and colorimetric method were used to detect the release of NO, TNOS and iNOS, and Real-time PCR method was used to detect expression changes of inflammatory genes such as TNF-α, IL-1β, IL-6 and IL-10. The results indicated that ACC at the mass concentration of 100 and 50 μg/mL could extremely significantly increase the survival rate of ST cells, and 25 μg/mL ACC could significantly increase the survival rate of ST cells. The content of NO increased sharply after TGEV infection, and the ACC at the mass concentration of 100 and 50 μg/mL could extremely significantly decrease the activities of TNOS and iNOS, and 25 μg/mL ACC could significantly decrease the activities of enzymes TNOS and iNOS. Meanwhile, ACC could extremely significantly down-regulate the expression levels of TNF-α, IL-1β, IL-6 and IL-10 mRNA. We can conclude that the alkaloids of Coptis chinensis could reduce the inflammatory response induced by TGEV and alleviate the damage of virus to cells.