Based on Yunyan87 testing material, we examined the influence of 15.0, 10.0, 5.0 μg/L concentrations of 8% diethyl aminoethyl hexanoate(DA-6) either singularly applied or applied in combination with calcium chloride on germination and seedling quality of flue-cured tobacco seeds. The results highlighted that: compared to the control treated with water, the emergence rate at 7th and 14th day after sowing were increased by 4.50%-10.40% and 2.74%-8.33% respectively, the seedling rate were increased by 3.19%-8.81% in the combined treatment, and in seedling period, seedling height, stem basal width, maximum leaf area were improved in the combined treatment by 5.99%-17.66%, 1.29%-5.47% and 21.1%-73.6% respectively; dry matter accumulation of root, stem, leaf, full stand of seeding and root-to-leaf ratio with combined treatment showed a progressive increase by 25.0%-55.0%, 16.7%-33.3%, 14.1%-33.8%, 16.5%-36.9% and 9.1%-17.0%, respectively, the content of leaf chlorophyll(chlorophyll a + chlorophyll b) with combined treatment was augmented by 26.0%-64.9%; the activity of nitrate reductase with combined treatment elevated by 52.1%-95.9%; catalase(CAT)activity, peroxidase(POD) activity, superoxide dismutase(SOD) enzyme activity and malondialdehyde (MDA) content with combined treatment were decreased by 41.5%-59.3%, 27.9%-62.9%, 30.3%-81.1% and 34.0%-63.7%, respectively. In conclusion, Yunyan87 seeds treated with 10.0 μg/L DA-6 in combination with 10.0 mmol/L calcium chloride showed the best effect.